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dc.contributor.authorGonzález García, Estefanía 
dc.contributor.authorGutiérrez Ulloa, Carlos Emilio 
dc.contributor.authorMata de la Mata, Francisco Javier de la 
dc.contributor.authorMarina Alegre, María Luisa 
dc.contributor.authorGarcía López, María Concepción 
dc.date.accessioned2020-09-30T08:25:31Z
dc.date.available2020-09-30T08:25:31Z
dc.date.issued2017-07-07
dc.identifier.bibliographicCitationAnalytical and Bioanalytical Chemistry, 2017, v. 409, n. 22, p. 5337-5348en
dc.identifier.issn1618-2642
dc.identifier.urihttp://hdl.handle.net/10017/44488en
dc.description.abstractReduction or removal of solvents and reagents in protein sample preparation is a requirement. Dendrimers can strongly interact with proteins and have great potential as a greener alternative to conventional methods used in protein sample preparation. This work proposes the use of single-walled carbon nanotubes (SWCNTs) functionalized with carbosilane dendrons with sulfonate groups for protein sample preparation and shows the successful application of the proposed methodology to extract proteins from a complex matrix. SEM images of nanotubes and mixtures of nanotubes and proteins were taken. Moreover, intrinsic fluorescence intensity of proteins was monitored to observe the most significant interactions at increasing dendron generations under neutral and basic pHs. Different conditions for the disruption of interactions between proteins and nanotubes after protein extraction and different concentrations of the disrupting reagent and the nanotube were also tried. Compatibility of extraction and disrupting conditions with the enzymatic digestion of proteins for obtaining bioactive peptides was also studied. Finally, sulfonate-terminated carbosilane dendron-coated SWCNTs enabled the extraction of proteins from a complex sample without using non-environmentally friendly solvents that were required so far.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)en
dc.rights© Springeren
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectSingle-walled carbon nanotubesen
dc.subjectSulfonate carbosilane dendronsen
dc.subjectDendron-coated nanotubesen
dc.subjectProtein-dendron interactionen
dc.subjectProtein extractionen
dc.titleSulfonate-terminated carbosilane dendron-coated nanotubes: a greener point of view in protein sample preparationen
dc.typeinfo:eu-repo/semantics/articleen
dc.subject.ecienciaQuímicaes_ES
dc.subject.ecienciaChemistryen
dc.contributor.affiliationUniversidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química
dc.contributor.affiliationUniversidad de Alcalá. Departamento de Química Orgánica y Química Inorgánica
dc.date.updated2020-09-30T08:24:52Z
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.identifier.doi10.1007/s00216-017-0479-3
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//CTQ-2014-54004-P//ESes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//AGL2012-36362/ES/VALORIZACION DE RESIDUOS SOLIDOS VEGETALES PROCEDENTES DE LA INDUSTRIA AGROALIMENTARIA/es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/CAM//S2013%2FABI-3028/ES/Estrategias avanzadas para la mejora y el control de la calidad y la seguridad de los alimentos/AVANSECAL-CMes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//AGL2016-79010-R/ES/DESARROLLO DE METODOS SOSTENIBLES PARA EL APROVECHAMIENTO DE SUBPRODUCTOS DE LA INDUSTRIA ALIMENTARIA CON ELEVADO CONTENIDO PROTEICOes_ES
dc.relation.projectID
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.uxxiAR/0000026753en
dc.identifier.publicationtitleAnalytical and Bioanalytical Chemistryen
dc.identifier.publicationvolume409
dc.identifier.publicationlastpage5348
dc.identifier.publicationissue22
dc.identifier.publicationfirstpage5337


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