Sulfonate-terminated carbosilane dendron-coated nanotubes: a greener point of view in protein sample preparation
Authors
González García, Estefanía; Gutiérrez Ulloa, Carlos Emilio; Mata de la Mata, Francisco Javier de la; Marina Alegre, María Luisa; García López, María ConcepciónIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/44488DOI: 10.1007/s00216-017-0479-3
ISSN: 1618-2642
Date
2017-07-07Affiliation
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química; Universidad de Alcalá. Departamento de Química Orgánica y Química InorgánicaBibliographic citation
Analytical and Bioanalytical Chemistry, 2017, v. 409, n. 22, p. 5337-5348
Keywords
Single-walled carbon nanotubes
Sulfonate carbosilane dendrons
Dendron-coated nanotubes
Protein-dendron interaction
Protein extraction
Project
info:eu-repo/grantAgreement/MINECO//CTQ-2014-54004-P//ES
info:eu-repo/grantAgreement/MINECO//AGL2012-36362/ES/VALORIZACION DE RESIDUOS SOLIDOS VEGETALES PROCEDENTES DE LA INDUSTRIA AGROALIMENTARIA/
info:eu-repo/grantAgreement/CAM//S2013%2FABI-3028/ES/Estrategias avanzadas para la mejora y el control de la calidad y la seguridad de los alimentos/AVANSECAL-CM
info:eu-repo/grantAgreement/MINECO//AGL2016-79010-R/ES/DESARROLLO DE METODOS SOSTENIBLES PARA EL APROVECHAMIENTO DE SUBPRODUCTOS DE LA INDUSTRIA ALIMENTARIA CON ELEVADO CONTENIDO PROTEICO
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© Springer
Access rights
info:eu-repo/semantics/openAccess
Abstract
Reduction or removal of solvents and reagents in protein sample preparation is a requirement. Dendrimers can strongly interact with proteins and have great potential as a greener alternative to conventional methods used in protein sample preparation. This work proposes the use of single-walled carbon nanotubes (SWCNTs) functionalized with carbosilane dendrons with sulfonate groups for protein sample preparation and shows the successful application of the proposed methodology to extract proteins from a complex matrix. SEM images of nanotubes and mixtures of nanotubes and proteins were taken. Moreover, intrinsic fluorescence intensity of proteins was monitored to observe the most significant interactions at increasing dendron generations under neutral and basic pHs. Different conditions for the disruption of interactions between proteins and nanotubes after protein extraction and different concentrations of the disrupting reagent and the nanotube were also tried. Compatibility of extraction and disrupting conditions with the enzymatic digestion of proteins for obtaining bioactive peptides was also studied. Finally, sulfonate-terminated carbosilane dendron-coated SWCNTs enabled the extraction of proteins from a complex sample without using non-environmentally friendly solvents that were required so far.
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