Sulfonate-terminated carbosilane dendron-coated nanotubes: a greener point of view in protein sample preparation
AuthorsGonzález García, Estefanía; Gutiérrez Ulloa, Carlos Emilio; Mata de la Mata, Francisco Javier de la; Marina Alegre, María Luisa; García López, María Concepción
IdentifiersPermanent link (URI): http://hdl.handle.net/10017/44488
AffiliationUniversidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química; Universidad de Alcalá. Departamento de Química Orgánica y Química Inorgánica
Analytical and Bioanalytical Chemistry, 2017, v. 409, n. 22, p. 5337-5348
Single-walled carbon nanotubes
Sulfonate carbosilane dendrons
AGL2012-36362, AGL2016-79010-R y CTQ-2014-54004-P (Ministerio de Economía y Competitividad de España)y financiación europea del programa FEDER (proyecto S2013 / ABI-3028, AVANSECAL-CM).
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Reduction or removal of solvents and reagents in protein sample preparation is a requirement. Dendrimers can strongly interact with proteins and have great potential as a greener alternative to conventional methods used in protein sample preparation. This work proposes the use of single-walled carbon nanotubes (SWCNTs) functionalized with carbosilane dendrons with sulfonate groups for protein sample preparation and shows the successful application of the proposed methodology to extract proteins from a complex matrix. SEM images of nanotubes and mixtures of nanotubes and proteins were taken. Moreover, intrinsic fluorescence intensity of proteins was monitored to observe the most significant interactions at increasing dendron generations under neutral and basic pHs. Different conditions for the disruption of interactions between proteins and nanotubes after protein extraction and different concentrations of the disrupting reagent and the nanotube were also tried. Compatibility of extraction and disrupting conditions with the enzymatic digestion of proteins for obtaining bioactive peptides was also studied. Finally, sulfonate-terminated carbosilane dendron-coated SWCNTs enabled the extraction of proteins from a complex sample without using non-environmentally friendly solvents that were required so far.
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