Stability and toxicity studies for duloxetine and econazole on Spirodela polyrhiza using chiral capillary electrophoresis
Authors
Marina Alegre, María Luisa; Valimaña Traverso, Jesús Manuel; Amariei, Georgiana; Boltes Espínola, Ana Karina; García González, María ÁngelesIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/41566DOI: 10.1016/j.jhazmat.2019.04.027
ISSN: 0304-3894
Date
2019-07-15Embargo end date
2020-07-16Affiliation
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaBibliographic citation
Journal of Hazardous Materials, 2019, v. 374, p. 203-210
Keywords
Aquatic plant
Chiral capillary electrophoresis
Duloxetine
Econazole
Mixture toxicity and stability
Project
Ministry of Economy and Competitiveness (Spain). Direccion General de Universidades e Investigacion de la Comunidad de Madrid (Spain). University of Alcala
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© Elsevier
Access rights
info:eu-repo/semantics/openAccess
Abstract
Stability and toxicity studies for duloxetine and econazole were achieved using individual solutions and their mixtures. Stability of drugs racemates and enantiomers was investigated under abiotic and biotic conditions. Toxicity was evaluated for the first time on Spirodela polyrhiza. EC50 values were calculated for each individual drug and for their binary mixture. Real (not nominal) concentrations determined by Capillary Electrophoresis were employed in the calculations of toxicity parameters. The use of a 25 mM phosphate buffer (pH 3.0) with 1.5% S-beta-CD as chiral selector at a temperature of 30 degrees C and a separation voltage of - 20 kV enabled the simultaneous enantiomeric separation of duloxetine and econazole in 7.5 min with enantiomeric resolutions of 7.9 and 6.5, respectively. For individual solutions, decay percentages under abiotic conditions were higher for duloxetine (80%) than for econazole (60%), while in presence of Spirodela polyrhiza they increased for duloxetine but not for econazole. Econazole showed the highest decay percentages under abiotic or biotic conditions (100%) in binary mixtures. EC50 values for duloxetine and econazole enabled to include both drugs within the group of very toxic compounds although econazole showed a higher toxicity than duloxetine and the binary mixture.
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Stability_Valimaña_JHazMat_2019.pdf | 1.422Mb |
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