Effect of the combined use of gamma-cyclodextrin and a chiral ionic liquid on the enantiomeric separation of homocysteine by capillary electrophoresis
Date
2018Affiliation
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química; Universidad de Alcalá. Departamento de Química Orgánica y Química InorgánicaBibliographic citation
Journal of Chromatography A, 2018, v. 1568, p. 222-228
Keywords
Capillary electrophoresis
chiral ionic liquids
cyclodextrins
homocysteine
enantioseparation
Description / Notes
doi: 10.1016/j.chroma.2018.07.023.
Project
CTQ2016-76368-P (Spanish Ministry of Economy and
337 Competitiveness)
CCG2016/EXP-071 (University of Alcalá)
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© Elsevier
Access rights
info:eu-repo/semantics/openAccess
Abstract
The enantioseparation of the non-protein amino acid homocysteine by CE was investigated in this article using seven neutral cyclodextrins and five chiral ionic liquids as chiral selectors. Using a previous derivatization step with FMOC and the subsequent separation under neutral conditions, homocysteine enantiomers were only separated when gamma-CD or (R)-N,N,N-trimethyl-2-aminobutanol-bis(trifluoromethane-sulfon)imidate (EtCholNTf2) were employed as sole chiral selectors in the separation buffer. On the one hand, gamma-CD gave rise to the enantiomeric separation in 10 min with a resolution value of 1.9, whereas EtCholNTf2 let to obtain a resolution value of 1.4 in more than 50 min. Then, the evaluation of the combined use of both selectors was also carried out, resulting in a considerable increase in the Rs. The best enantioseparation for homocysteine was obtained when 10 mM EtCholNTf2 was added to 50 mM phosphate buffer (pH 7.0) containing 2 mM gamma-CD. In an attempt to discriminate specific chiral cation effect from the salt effect, the influence of adding LiNTf2 to the separation medium was also evaluated, resulting in lower resolution values for homocysteine when compared to those achieved with the addition of EtCholNTf2, indicating a synergistic effect between EtCholNTf2 and gamma-CD. Interestingly, the enantiomer migration order changed depending on the chiral selector used. When EtCholNTf2 or gamma-CD were used as sole chiral selectors, D-enantiomer was the first-migrating enantiomer. However, an inversion in the migration order was observed when both selectors were employed in a dual system being the L- enantiomer the first-migrating one.
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