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dc.contributor.authorMartínez Girón, Ana Belén 
dc.contributor.authorDomínguez Vega, Elena 
dc.contributor.authorGarcía Ruiz, Carmen 
dc.contributor.authorCrego Navazo, Antonio Luis 
dc.contributor.authorMarina Alegre, María Luisa 
dc.date.accessioned2009-09-08T11:21:58Z
dc.date.available2009-09-08T11:21:58Z
dc.date.issued2008
dc.identifier.bibliographicCitationJournal of Chromatography B, 2008, v. 875, p. 254-259en
dc.identifier.issn1570-0232
dc.identifier.urihttp://hdl.handle.net/10017/3831
dc.description.abstractA new analytical methodology was developed by EKC enabling the fast enantiomeric separation of Ornithine in complex mixtures of amino acids. A previous derivatization step with 6-aminoquinolyl-Nhydroxysuccinimidyl carbamate (AQC) was achieved to enable the sensitive UV detection of amino acids as well as to make possible their interaction with the CDs employed as chiral selectors. A dual CD system containing an anionic and a neutral CD in phosphate buffer at acid pH showed a high resolving power allowing the enantiomeric separation of 18 protein amino acids and Orn. The method was applied to the analysis of fermented foods to investigate the extent of the presence of Orn enantiomers.en
dc.description.sponsorshipAuthors thank the Ministry of Education of Science (Spain) and the Comunidad Autonoma de Madrid (Spain) for research projects CTQ2006-03849/BQU and S-0505/AGR-0312, respectively. Carmen Garcıa-Ruiz thanks the Ministry of Science and Technology (Spain) for the Ramon y Cajal program (RYC-2003-001). Ana Belen Martínez Giron and Elena Domínguez-Vega thank the University of Alcala for their pre-doctoral grants.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherB. Chankvetadze and E. Francotteen
dc.publisherElsevieren
dc.rights(C) Elsevier, 2008en
dc.subjectOrnithineen
dc.subjectChiral separationen
dc.subjectCyclodextrinsen
dc.subjectAQC derivatizationen
dc.subjectAmino acidsen
dc.subjectFermented foodsen
dc.subjectElectrokinetic chromatographyen
dc.titleEnantiomeric separation of ornithine in complex mixtures of amino acids by EKC with off-line derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamateen
dc.typeinfo:eu-repo/semantics/articleen
dc.subject.ecienciaCienciaes_ES
dc.subject.ecienciaQuímica analítica e industriales_ES
dc.subject.ecienciaScienceen
dc.subject.ecienciaChemistry, analytic and technicalen
dc.contributor.affiliationUniversidad de Alcalá. Departamento de Química Analítica e Ingeniería Químicaes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.jchromb.2008.06.022
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.identifier.doi10.1016/j.jchromb.2008.06.022
dc.relation.projectIDinfo:eu-repo/grantAgreement/MEC//CTQ2006-03849/ES/Nuevas Contribuciones para el Desarrollo de Metodologías Analíticas de Alta Sensibilidad y Resolución Mediante Técnicas Microseparativas/es_ES
dc.relation.projectIDS-0505/AGR-0312 (Comunidad de Madrid)
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//RYC-2013-12688/ES/RYC-2013-12688/es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen


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