dc.contributor.author | Martínez Girón, Ana Belén | |
dc.contributor.author | Domínguez Vega, Elena | |
dc.contributor.author | García Ruiz, Carmen | |
dc.contributor.author | Crego Navazo, Antonio Luis | |
dc.contributor.author | Marina Alegre, María Luisa | |
dc.date.accessioned | 2009-09-08T11:21:58Z | |
dc.date.available | 2009-09-08T11:21:58Z | |
dc.date.issued | 2008 | |
dc.identifier.bibliographicCitation | Journal of Chromatography B, 2008, v. 875, p. 254-259 | en |
dc.identifier.issn | 1570-0232 | |
dc.identifier.uri | http://hdl.handle.net/10017/3831 | |
dc.description.abstract | A new analytical methodology was developed by EKC enabling the fast enantiomeric separation of
Ornithine in complex mixtures of amino acids. A previous derivatization step with 6-aminoquinolyl-Nhydroxysuccinimidyl
carbamate (AQC) was achieved to enable the sensitive UV detection of amino acids
as well as to make possible their interaction with the CDs employed as chiral selectors. A dual CD system
containing an anionic and a neutral CD in phosphate buffer at acid pH showed a high resolving power
allowing the enantiomeric separation of 18 protein amino acids and Orn. The method was applied to the
analysis of fermented foods to investigate the extent of the presence of Orn enantiomers. | en |
dc.description.sponsorship | Authors thank the Ministry of Education of Science (Spain) and
the Comunidad Autonoma de Madrid (Spain) for research projects CTQ2006-03849/BQU and S-0505/AGR-0312, respectively. Carmen Garcıa-Ruiz thanks the Ministry of Science and Technology (Spain) for the Ramon y Cajal program (RYC-2003-001). Ana Belen Martínez
Giron and Elena Domínguez-Vega thank the University of Alcala for their pre-doctoral grants. | en |
dc.description.sponsorship | Ministerio de Educación y Cultura | es_ES |
dc.description.sponsorship | Comunidad de Madrid | es_ES |
dc.description.sponsorship | Ministerio de Economía, Comercio y Empresa | es_ES |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | B. Chankvetadze and E. Francotte | en |
dc.publisher | Elsevier | en |
dc.rights | (C) Elsevier, 2008 | en |
dc.subject | Ornithine | en |
dc.subject | Chiral separation | en |
dc.subject | Cyclodextrins | en |
dc.subject | AQC derivatization | en |
dc.subject | Amino acids | en |
dc.subject | Fermented foods | en |
dc.subject | Electrokinetic chromatography | en |
dc.title | Enantiomeric separation of ornithine in complex mixtures of amino acids by EKC with off-line derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate | en |
dc.type | info:eu-repo/semantics/article | en |
dc.subject.eciencia | Ciencia | es_ES |
dc.subject.eciencia | Química analítica e industrial | es_ES |
dc.subject.eciencia | Science | en |
dc.subject.eciencia | Chemistry, analytic and technical | en |
dc.contributor.affiliation | Universidad de Alcalá. Departamento de Química Analítica e Ingeniería Química | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.jchromb.2008.06.022 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dc.identifier.doi | 10.1016/j.jchromb.2008.06.022 | |
dc.relation.projectID | info:eu-repo/grantAgreement/MEC//CTQ2006-03849/ES/Nuevas Contribuciones para el Desarrollo de Metodologías Analíticas de Alta Sensibilidad y Resolución Mediante Técnicas Microseparativas/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/CAM/Proyecto de Excelencia Científica/S-0505-AGR-0312/ES/Nuevas metodologías analíticas para el estudio y control de seguridad y calidad de los alimentos/ANALISTYC-CM) | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//RYC-2013-12688/ES/DEVELOPMENT OF ADVANCED ANALYTICAL METHODOLOGIES FOR FOOD ANALYSIS TO IDENTIFY AND QUANTIFY CHIRAL AND NON CHIRAL BIOMARKERS OF FOOD QUALITY, SAFETY AND TRACEABILITY | en |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |