Enantiomeric separation of ornithine in complex mixtures of amino acids by EKC with off-line derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate
AuthorsMartínez Girón, Ana Belén; Domínguez Vega, Elena; García Ruiz, Carmen; Crego Navazo, Antonio Luis; Marina Alegre, María Luisa
IdentifiersPermanent link (URI): http://hdl.handle.net/10017/3831
B. Chankvetadze and E. Francotte
Authors thank the Ministry of Education of Science (Spain) and the Comunidad Autonoma de Madrid (Spain) for research projects CTQ2006-03849/BQU and S-0505/AGR-0312, respectively. Carmen Garcıa-Ruiz thanks the Ministry of Science and Technology (Spain) for the Ramon y Cajal program (RYC-2003-001). Ana Belen Martínez Giron and Elena Domínguez-Vega thank the University of Alcala for their pre-doctoral grants.
Journal of Chromatography B, 2008, v. 875, p. 254-259
CTQ2006-03849/BQU (Ministerio de Educación y Ciencia)
S-0505/AGR-0312 (Comunidad de Madrid)
RYC-2003-001 (Ministerio de Ciencia y Tecnología)
(C) Elsevier, 2008
A new analytical methodology was developed by EKC enabling the fast enantiomeric separation of Ornithine in complex mixtures of amino acids. A previous derivatization step with 6-aminoquinolyl-Nhydroxysuccinimidyl carbamate (AQC) was achieved to enable the sensitive UV detection of amino acids as well as to make possible their interaction with the CDs employed as chiral selectors. A dual CD system containing an anionic and a neutral CD in phosphate buffer at acid pH showed a high resolving power allowing the enantiomeric separation of 18 protein amino acids and Orn. The method was applied to the analysis of fermented foods to investigate the extent of the presence of Orn enantiomers.
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