Capsaicin Targets Lipogenesis in HepG2 Cells Through AMPK Activation, AKT Inhibition and PPARs Regulation
Authors
Bort Bueno, Alicia Carmen; Sánchez Gómez, Belén; Mateos Gómez, Pedro Antonio; Díaz-Laviada Marturet, Inés; Rodríguez Henche, NievesIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/37531DOI: 10.3390/ijms20071660
ISSN: 1422-0067
Date
2019Funders
Fundación Tatiana Pérez De Guzmán
Bibliographic citation
International Journal of Molecular Sciences, 2019, v. 20(7), n. 1660, p. -
Keywords
Capsaicin
AMPK
AKT
PPARy
PPARx
HepG2
Lipid metabolism
Project
info:eu-repo/grantAgreement/Fundación Tatiana Pérez de Guzmán//2013-001/ES
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution 4.0 International (CC BY 4.0)
© 2019 The Author(s)
Access rights
info:eu-repo/semantics/openAccess
Abstract
Obesity, a major risk factor for chronic diseases such as type 2 diabetes (T2D), represents a serious primary health problem worldwide. Dietary habits are of special interest to prevent and counteract the obesity and its associated metabolic disorders, including lipid steatosis. Capsaicin, a pungent compound of chili peppers, has been found to ameliorate diet-induced obesity in rodents and humans. The purpose of this study was to examine the effect of capsaicin on hepatic lipogenesis and to delineate the underlying signaling pathways involved, using HepG2 cells as an experimental model. Cellular neutral lipids, stained with BODIPY493/503, were quantified by flow cytometry, and the protein expression and activity were determined by immunoblotting. Capsaicin reduced basal neutral lipid content in HepG2 cells, as well that induced by troglitazone or by oleic acid. This effect of capsaicin was prevented by dorsomorphin and GW9662, pharmacological inhibitors of AMPK and PPARgamma, respectively. In addition, capsaicin activated AMPK and inhibited the AKT/mTOR pathway, major regulators of hepatic lipogenesis. Furthermore, capsaicin blocked autophagy and increased PGC-1alfa protein. These results suggest that capsaicin behaves as an anti-lipogenic compound in HepG2 cells.
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capsaicin_bort_IJMS_2019.pdf | 2.132Mb |
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capsaicin_bort_IJMS_2019.pdf | 2.132Mb |
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