dc.contributor.author | Gómez Ramírez, Rafael | |
dc.contributor.author | Martínez Martínez, Ángel | |
dc.contributor.author | Fuentes Paniagua, María Elena | |
dc.contributor.author | Baeza García, Alejandro | |
dc.contributor.author | Mata de la Mata, Francisco Javier de la | |
dc.contributor.author | Sánchez-Nieves Fernández, Javier | |
dc.contributor.author | Ciuendez, Monica | |
dc.contributor.author | González Mancebo, Blanca | |
dc.contributor.author | Vallet Reig, Maria | |
dc.date.accessioned | 2018-07-27T09:04:19Z | |
dc.date.available | 2018-07-27T09:04:19Z | |
dc.date.issued | 2015-09-10 | |
dc.identifier.bibliographicCitation | Chemistry - A European Journal, 2015, v. 21, p. 1-17 | en |
dc.identifier.issn | 0947-6539 | |
dc.identifier.uri | http://hdl.handle.net/10017/33947 | |
dc.description.abstract | A novel nanosystem based on mesoporous silica nanoparticles covered with carbosilane dendrons grafted on their external surface is reported. This system is able to transport single oligonucleotide strands into cells, avoiding the electrostatic repulsion between the cell membrane and the negatively charged nucleic acids thanks to the cationic charge provided by the dendron coating in physiological conditions. Moreover, the presence of the highly ordered pore network inside the silica matrix would make possible to allocate other therapeutic agents within the mesopores with the aim of achieving a double delivery. First, carbosilane dendrons of second and third generation pos- sessing ammonium or tertiary amine groups as peripheral functional groups were prepared. Hence, different strategies were tested in order to obtain their suitable grafting on the nanoparticles outer surface. As nucleic acid model, a single stranded DNA oligonucleotide tagged with a fluorescent Cy3 moiety was used to evaluate the DNA adsorption capacity. The hybrid material functionalized with the third generation of neutral dendron showed excellent DNA binding properties. Finally, the cytotoxicity as well as the capability to deliver DNA into cells, was tested using a human osteoblast-like cell line, achieving good levels of internalization of the vector DNA/carbosilane dendron functionalized material without affecting cellular viability. | en |
dc.description.sponsorship | Ministerio de Economía y Empresa | es-ES |
dc.description.sponsorship | Comunidad de Madrid | es_ES |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | Wiley | en |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) | en |
dc.rights | (c) Wiley, 2015 | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | Carbosilane dendrons | en |
dc.subject | Mesoporous silica nanoparticles | en |
dc.subject | Gene transfection | en |
dc.subject | Nonviral vectors | en |
dc.title | Mesoporous Silica Nanoparticles Decorated with Carbosilane Dendrons as New Non-viral Oligonucleotide Delivery Carriers | en |
dc.type | info:eu-repo/semantics/article | en |
dc.subject.eciencia | Química | es_ES |
dc.subject.eciencia | Chemistry | en |
dc.contributor.affiliation | Universidad de Alcalá. Departamento de Química Orgánica y Química Inorgánica | es_ES |
dc.date.updated | 2018-07-27T08:40:41Z | |
dc.type.version | info:eu-repo/semantics/acceptedVersion | en |
dc.identifier.doi | 10.1002/chem.201501966 | |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//MAT2012-35556/ES/BIOCERÁMICAS DE SÍLICE MESOPOROSA CON PROPIEDADES ESTÍMULO-RESPUESTA PARA EL TRATAMIENTO SECUENCIAL DE PATOLOGÍAS DEL TEJIDO ÓSEO | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/CAM//S2011%2FBMD-2351/ES/Nanosistemas Dendríticos para Aplicaciones Biomédicas - Dendritic Nanosystems for Biomedical Aplications | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |
dc.identifier.uxxi | AR/0000022494 | |
dc.identifier.publicationtitle | Chemistry - A European Journal | en |
dc.identifier.publicationvolume | 21 | |
dc.identifier.publicationlastpage | 17 | |
dc.identifier.publicationfirstpage | 1 | |