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dc.contributor.authorMarina Alegre, María Luisa es_ES
dc.contributor.authorGarcía López, María Concepción es_ES
dc.contributor.authorVásquez Villanueva, Romyes_ES
dc.date.accessioned2016-03-16T12:30:54Z
dc.date.available2017-07-12T02:45:06Z
dc.date.issued2016
dc.identifier.bibliographicCitationJournal of Chromatography A, 2016, v.1428, n.8, p.185-192en
dc.identifier.issn0021-9673
dc.identifier.urihttp://hdl.handle.net/10017/24608
dc.description.abstractHILIC- and RP-HPLC-ESI-Q-TOF identification of bioactive peptides with antioxidant capacity in peach by-products was carried out. Peach seeds contain more than 40% of proteins (as dried and defatted basis) and could constitute a cheap source of bioactive peptides. Extracted proteins were digested using four different commercial enzymes. Five assays based on different antioxidant mechanisms were employed for a reliable evaluation of the antioxidant capacity of the extracts. Thermolysin enzyme originated the extract with the most favorable antioxidant capacity. Probably due to a synergic effect among antioxidant peptides, it was not possible to find a peptide fraction with a higher antioxidant capacity than the whole extract. Eighteen peptides were identified in the whole hydrolysate when combining HILIC- and RP-HPLC-ESI-Q-TOF. A high percentage of hydrophobic amino acids were observed within their sequences which is a characteristic feature of the antioxidant nature of peptides.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherElsevieren
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Españaes_ES
dc.rights© Elsevier, 2015en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectHydrophilic interactionen
dc.subjectReversed-phaseen
dc.subjectLiquid chromatography–tandem mass spectrometryen
dc.subjectPeptidesen
dc.subjectAntioxidanten
dc.subjectPeach seeden
dc.titleIdentification by hydrophilic interaction and reversed-phase liquid chromatography-tandem mass spectrometry of peptides with antioxidant capacity in food residuesen
dc.typeinfo:eu-repo/semantics/articleen
dc.subject.ecienciaCIENCIAes_ES
dc.subject.ecienciaQuímicaes_ES
dc.subject.ecienciaSCIENCEen
dc.subject.ecienciaChemistryen
dc.contributor.affiliationUniversidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química. Unidad docente de Química Analítica e Ingeniería Químicaes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.chroma.2015.07.032
dc.type.versioninfo:eu-repo/semantics/acceptedVersionen
dc.identifier.doi10.1016/j.chroma.2015.07.032
dc.relation.projectIDAGL2012-36362 (Ministerio de Economía y Competitividad)
dc.relation.projectIDS2013/ABI-3028/AVANSECAL (Comunidad de Madrid)
dc.relation.projectIDCCG2013/EXP-028 (Universidad de Alcalá)
dc.date.embargoEndDate2017-07-12
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen


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