Fractionation and identification of antioxidant and angiotensin-converting enzyme-inhibitory peptides obtained from plum (Prunus domestica L.) stones
Authors
Marina Alegre, María LuisaIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/24159DOI: 10.1016/j.jff.2015.08.033
ISSN: 1756-4646
Publisher
Elsevier
Date
2015Embargo end date
2016-12-01Funders
Novozymes Spain S.A.
Bibliographic citation
Journal of Functional Foods, 2015, v.19, n.A, p.376–384
Keywords
Plum stone
ACE-inhibitory peptides
Antioxidant peptides
Hydrophilic interaction chromatography
Mass spectrometry
Project
info:eu-repo/grantAgreement/MINECO//AGL2012-36362/ES/VALORIZACION DE RESIDUOS SOLIDOS VEGETALES PROCEDENTES DE LA INDUSTRIA AGROALIMENTARIA/
CCG2013/EXP-028 (Universidad de Alcalá)
S2013/ABI-3028/AVANSECAL (Comunidad de Madrid)
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/acceptedVersion
Publisher's version
http://dx.doi.org/10.1016/j.jff.2015.08.033Rights
© Elsevier, 2015
Access rights
info:eu-repo/semantics/openAccess
Abstract
Peptides with antioxidant and ACE-inhibitory capacities have been obtained from a plum processing by-product. The aim of this work was to fractionate most active plum hydrolysates, to identify peptides in these fractions, and to evaluate their resistance to gastrointestinal enzymes. Highest antioxidant capacity was observed in the whole Alcalase hydrolysate while peptides yielding the highest ability to inhibit ACE were concentrated in the fraction <3 kDa from the Thermolysin hydrolysate. RP-HPLC–ESI-MS/MS enabled the identification of 13 and 7 potential antioxidant and ACE inhibitory peptides, respectively, in these fractions. Nevertheless, RP-HPLC alone did not guarantee the successful separation of all peptides. The combination of RP-HPLC and hydrophilic interaction liquid chromatography (HILIC) coupled to ESI-Q-TOF-MS/MS enabled the identification of all peptides. Five potential antioxidant and three potential antihypertensive peptides could be additionally identified by HILIC. A total of 7 potential antioxidant peptides and 3 potential antihypertensive peptides resisted a simulated gastrointestinal digestion.
Files in this item
Files | Size | Format |
|
---|---|---|---|
Fractionation_identification_J ... | 682.7Kb |
![]() |
Files | Size | Format |
|
---|---|---|---|
Fractionation_identification_J ... | 682.7Kb |
![]() |
Collections
- QUANING - Artículos [257]