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dc.contributor.authorLópez Sañudo, Susana
dc.contributor.authorRodríguez Martín, Eulalia 
dc.contributor.authorMartín Espinosa, Ángela
dc.contributor.authorArilla Ferreiro, Eduardo 
dc.date.accessioned2008-11-24T12:38:46Z
dc.date.available2008-11-24T12:38:46Z
dc.date.issued1994
dc.identifier.bibliographicCitationPeptides, 1995, v. 16, n. 8, p. 1453-1459en
dc.identifier.issn0196-9781
dc.identifier.urihttp://hdl.handle.net/10017/2316
dc.description.abstractSomatostatin (SS) and noradrenaline (NA) are distributed in the rat cerebral cortex, and seizure activity is one of the aspects of behavior affected by both neurotransmitters. Due to the possible interaction between both neurotransmitter systems, we studied whether phenylphrine, an alpha(1)-adrenoceptor agonist, and prazosin, an alpha(1)-adrenoceptor antagonist, can modulate SS-like immunoreactivity (SS-LI) levels, binding of [I-125][Tyr(11)]SS to its specific receptors, the ability of SS to inhibit adenylate cyclase (AC) activity, and the guanine nucleotide binding regulatory protein G(1) and G(0), in the Sprague-Dawley rat frontoparietal cortex. An IP dose of 2 or 4 mg/kg of phenylephrine injected 7 h before decapitation decreased the number of SS receptors and increased the apparent affinity in frontoparietal cortex membranes. An IP dose of 20 or 25 mg/kg of prazosin administered 8 h before decapitation increased the number of SS receptors and decreased their apparent affinity. The administration of prazosin before the phenylephrine injection prevented the phenylephrine-induced changes in SS binding. The addition of phenylephrine and/or prazosin 10(-5) M to the incubation medium changed neither the number nor the affinity of the SS receptors in the frontoparietal cortex membranes. Phenylephrine or prazosin affected neither SS-LI content nor the basal or forskolin (FK)-stimulated AC activities in the frontoparietal cortex. In addition, SS caused an equal inhibition of AC activity in frontoparietal cortex membranes of phenylephrine- and prazosin-treated rats compared with the respective control group. Finally, phenylephrine and prazosin did not vary the pertussis toxin (PTX)-catalyzed ADP ribosylation of G(1)- and/or G(0)-proteins. These results suggest that the above mentioned changes are related to the phenylephrine activation of alpha(1)-adrenoceptors or to the blocking of these receptors by prazosin. In addition, these data provide further support for a functional interrelationship between the alpha(1)-adrenergic and somatostatinergic systems in the rat frontoparietal cortex.en
dc.description.sponsorshipMinisterio de Educación y Cienciaes_ES
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherElsevieren
dc.rights(c) Elsevier Science, 1995en
dc.subjectPhenylephrineen
dc.subjectPrazosinen
dc.subjectSomatostatin receptorsen
dc.subjectAdenylate cyclaseen
dc.subjectG-proteinsen
dc.subjectFrontoparietal cortexen
dc.titleEffect of phenylephrine and prazosin on the somatostatinergic system in the rat frontoparietal cortexen
dc.typeinfo:eu-repo/semantics/articleen
dc.subject.ecienciaBioquímicaes_ES
dc.subject.ecienciaBiochemistryen
dc.subject.ecienciaScienceen
dc.subject.ecienciaCienciaes_ES
dc.contributor.affiliationUniversidad de Alcalá. Departamento de Bioquímica y Biología Molecular
dc.relation.publisherversionhttp://dx.doi.org/10.1016/0196-9781(95)02011-X
dc.type.versioninfo:eu-repo/semantics/acceptedVersionen
dc.identifier.doi10.1016/0196-9781(95)02011-X
dc.relation.projectIDinfo:eu-repo/grantAgreement/MEC//PB94-0339/ES//es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen


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