Influence of fluoxetine and p-chloroamphetamine on the somatostatin receptor-adenylyl cyclase system in the rat frontoparietal cortex
Authors
Muñoz Acedo, Gema; Izquierdo Claros, Rosa María; Puebla Jiménez, Lilian; Rodríguez Martín, Eulalia; Arilla Ferreiro, EduardoIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/2260DOI: 10.1016/S0169-328X(97)00038-7
ISSN: 0169-328X
Publisher
Elsevier
Date
1997Funders
Ministerio de Educación y Cultura
Universidad de Alcalá
Bibliographic citation
Molecular Brain Research, 1997, v. 47, p. 117–124
Keywords
Fluoxetine
p-Chloroamphetamine
Somatostatin receptor
Adenylyl cyclase
Rat
Frontoparietal cortex
Project
info:eu-repo/grantAgreement/MEC//PM95-0041/ES/EFECTO DEL OXIDO NITRICO SOBRE EL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA EN EL CEREBRO HUMANO Y DE RATA
info:eu-repo/grantAgreement/UAH//001%2F96/ES/REGULACION DEL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA POR EL NEUROPEPTIDO Y (NPY) EN EL CEREBRO DE LA RATA
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Publisher's version
http://dx.doi.org/10.1016/S0169-328X(97)00038-7Rights
(c) Elsevier Science, 1997
Access rights
info:eu-repo/semantics/openAccess
Abstract
There is evidence that suggests a reciprocal functional link between the serotonergic and the somatostatinergic system in the rat frontoparietal cortex. However, to date, the role of endogenous 5-hydroxytryptamine (serotonin) on the regulation of the somatostatin (SS) receptor-adenylyl cyclase (AC) system remains unclear. In the present study, the administration of fluoxetine (10 mg/kg i.p.), a 5-hydroxytryptamine uptake inhibitor in a single dose or administered daily for 14 days increased the number of specific [125I]Tyr11-SS receptors, with no change in the receptor affinity, in rat frontoparietal cortical membranes. However, the capacity of SS to inhibit forskolin (FK)-stimulated AC activity in these membranes was lower than in the control groups. The ability of the stable GTP analogue 5'-guanylylimidodiphosphate (Gpp(NH)p) to inhibit FK-stimulated AC activity in frontoparietal cortical membranes was also decreased in rats acutely and chronically treated with fluoxetine. p-Chloroamphetamine (5 mg/kg i.p.), which leads to a lasting reduction of 5-hydroxytryptamine innervation, administered,on days 1, 3 and 5 and the rats sacrificed 1 or 3 weeks after the first injection, decreased the number of SS receptors without changing the receptor affinity. In this experimental group, SS also caused a significantly lower inhibition of FK-stimulated AC activity. p-Chloroamphetamine had no effect on the ability of Gpp(NH)p to inhibit FK-stimulated AC activity in frontoparietal cortical membranes at all the time periods studied. The present results suggest that under normal circumstances some SS receptors are under a tonic stimulatory control through the serotonergic system.
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