Determination of non-protein amino acids and betaines in vegetable oils by flow injection triple-quadrupole tandem mass spectrometry: a screening method for the detection of adulterations of olive oils.

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Authors
Sánchez Hernández, LauraUniversity of Alcalá Author; Nozal Martínez, LeonorUniversity of Alcalá Author; Marina Alegre, María LuisaUniversity of Alcalá Author; Crego Navazo, Antonio LuisUniversity of Alcalá Author
Identifiers
Permanent link (URI): http://hdl.handle.net/10017/21129
DOI: 10.1021/jf202857y
ISSN: 0021-8561
Publisher
American Chemical Society
Date
2012
Academic Departments
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química
Teaching unit
Unidad docente Química Analítica e Ingeniería Quimica
Funders
The projects involved in this work have been: project CTQ2009-09022 from the Spanish Ministry of Science and Innovation (Spain), project S2009/AGR-1464 from the Comunidad Autónoma of Madrid (Spain) and European funding from FEDER programme (ANALISYC-II), and project CCG10-UAH/AGR-5950 from the University of Alcalá and the Comunidad Autónoma of Madrid (Spain
Comunidad de Madrid
Ministerio de Ciencia e Innovación
Bibliographic citation
Journal of Agricultural and Food Chemistry, 2012, v. 60, n. 4, p. 896-903
Keywords
Adulteration
Betaines
Non-protein amino acids
Screening
Tandem mass spectrometry
Vegetable oils
Description / Notes
This document is the unedited author's version of a Submitted Work that was subsequently accepted for publication in Journal of Agricultural and Food Chemistry, copyright © American Chemical Society after peer review.
Project
info:eu-repo/grantAgreement/CAM//CCG10-UAH/AGR-5950/ES/METODOLOGÍAS ANALÍTICAS AVANZADAS POR ESPECTROMETRÍA DE MASAS ACOPLADA O NO A TÉCNICAS SEPARATIVAS EN FASE LÍQUIDA (LC/CE) PARA LA DETERMINACIÓN DE PEQUEÑAS Y GRANDES MOLÉCULAS EN EL CONTROL DE LA CALIDAD DE LOS ALIMENTOS
info:eu-repo/grantAgreement/MICIN//CTQ2009-09022//ES/NUEVAS ESTRATEGIAS ANALÍTICAS PARA LA DETERMINACIÓN DE ENANTIOMEROS Y PEPTIDOS BIOACTIVOS POR MICRO/NANO TÉCNICAS
info:eu-repo/grantAgreement/CAM//S2009/AGR-1464/ES/METODOLOGÍAS ANALÍTICAS INNOVADORAS PARA EL CONTROL DE LA CALIDAD Y LA SEGURIDAD DE LOS ALIMENTOS/ANALISYC-II
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/acceptedVersion
Publisher's version
http://dx.doi.org/10.1021/jf202857y
Rights
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Access rights
info:eu-repo/semantics/openAccess
Abstract
A novel screening method using an automated flow injection electrospray ionization tandem mass spectrometry system is proposed for the simultaneous determination of five nonprotein amino acids (β-alanine, alloisoleucine, ornithine, citrulline, pyroglutamic acid) and three betaines (glycine betaine, trigonelline, proline betaine) after derivatization with butanolic HCl. MS/MS experiments were carried out in a triple-quadrupole instrument using multiple reaction monitoring mode in <2 min. The proposed method provided high fingerprinting power to identify the presence of five of the studied compounds in different types of vegetable oils (soybean, sunflower, corn, olive) with LODs at parts per billion levels. The method was validated, and different mixtures of extra virgin olive oil with seed oils were analyzed, achieving the typification for the detection of adulterations in extra virgin olive oils up to 2% w/w. The nonprotein amino acid ornithine was confirmed as a marker for adulteration in the olive oils analyzed

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