Cell-based high-throughput screening assay for identification of G-protein-coupled receptors agonists and antagonists
Authors
Bejarano Prudencio, AlbertoPublisher
InnovARTE, Grupo Docente. Universidad de Alcalá
Date
2013Bibliographic citation
A. Bejarano (2013) Cell-Based High-Throughput Screening Assay for Identification of G-Protein-Coupled Receptors Agonists and Antagonists. Dianas 2(1): e20130302. ISSN 1886-8746
Keywords
Ca2+ mobilization
GPCRs
FLIPR
Fluorescent Ca2+ dye
High-throughput screening
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/submittedVersion
Access rights
info:eu-repo/semantics/openAccess
Abstract
The GPCRs are the target of a significant portion of all approved drugs and also represent an important parcel of potential drug targets for new indications. Activation of GPCRs can be detected by the utilization of functional cell-based assays like measuring transient intracellular calcium mobilization. The emergence of the fluorescent imaging plate reader and Ca2+-sensitive fluorescent dyes has made the high-content screening of GPCR possible. Here was applied a dual automated FLIPR platform to testing a large collection of compounds to identification of both agonist and antagonists form a single screen of GPCR
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Files | Size | Format |
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cell_bejarano_DIANAS_2013.pdf | 669.1Kb |
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