Fast enantiomeric separation of uniconazole and diniconazole by electrokinetic chromatography using an anionic cyclodextrin: application to the determination of analyte-selector apparent binding constants for enantiomers
Identificadores
Enlace permanente (URI): http://hdl.handle.net/10017/1366DOI: 10.1002/1522-2683(20000901)21:15<3240::AID-ELPS3240>3.0.CO;2-T
ISSN: 0173-0835
Editor
John Wiley & Sons
Fecha de publicación
2000Cita bibliográfica
Electrophoresis, 2000, v. 21, p. 3240-3248
Palabras clave
Uniconazole
Diniconazole
Electrokinetic chromatography
Proyectos
07M-0049/1998 (Comunidad de Madrid)
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/publishedVersion
Derechos
(c) WILEY-VCH, 2000
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
The enantiomeric resolution of the fungicides uniconazole and diniconazole was performed using electrokinetic chromatography with cyclodextrins as pseudostationary phase (CD-EKC). A systematic evaluation of several chiral selectors was made. The anionic derivative carboxymethylated-gamma-cyclodextrin (CM-gamma-CD) was found to be the most appropriate for the enantioseparation of fungicides among all cyclodextrins tested. The influence of some experimental conditions such as nature and buffer pH, chiral selector concentration, and temperature on the enantiomeric separation of the compounds studied was also investigated. The use of a 50 mM phosphate buffer (pH 6.5) containing 5 mM CM-gamma-CD and a temperature of 50 degrees C enabled the baseline enantioresolution of mixtures of uniconazole and diniconazole in less than 5 min. In addition, apparent binding constants for each enantiomer-CM-gamma-CD pair at several temperatures, as well as thermodynamic parameters for binding were calculated.
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UniDini-Electrophoresis, 21, ... | 138.9Kb |
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UniDini-Electrophoresis, 21, ... | 138.9Kb |
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