dc.contributor.author | García Ruiz, Carmen | |
dc.contributor.author | Torre Roldán, María Mercedes | |
dc.contributor.author | Marina Alegre, María Luisa | |
dc.date.accessioned | 2008-03-03T09:50:46Z | |
dc.date.available | 2008-03-03T09:50:46Z | |
dc.date.issued | 1999 | |
dc.identifier.bibliographicCitation | Journal of Chromatography A, 1999, v. 859, p. 77-86 | en |
dc.identifier.issn | 0021-9673 | |
dc.identifier.uri | http://hdl.handle.net/10017/1363 | |
dc.description.abstract | The simultaneous separation of bovine whey proteins [a-lactalbumin and b-lactoglobulin (A1B)] and soybean proteins
was performed, for the first time, by capillary electrophoresis. Different experimental conditions were tested. The most
suitable consisted of 0.050 M phosphate buffer (pH 8) with 1 M urea and 1.2 mg/ml methylhydroxyethylcellulose, UV
detection at 280 nm, 15 kV applied voltage, and 308C temperature. Quantitation of bovine whey proteins in a commercial
powdered soybean milk manufactured by adding bovine whey to its formulation was performed using the calibration method
of the external standard. Direct injection of a solution of the powdered soybean milk only enabled quantitation of
a-lactalbumin in the commercial sample. Detection of b-lactoglobulin (A1B) required acid precipitation of the solution of
the sample in order to concentrate bovine whey proteins in the supernatant prior to the analysis of this protein in the whey
obtained. Since a-lactalbumin could also be quantitated from the injection of the whey, the simultaneous determination of
a-lactalbumin and b-lactoglobulin (A1B) was possible upon acid precipitation of the powdered soybean milk solution.
Detection limits obtained were 14 mg/g sol. for a-lactalbumin and 52 mg/g sol. for b-lactoglobulin (A1B) which represent
protein concentrations about 60 mg/100 g sample for a-lactalbumin and 100 mg/100 g sample for b-lactoglobulin (A1B).
1999 Elsevier Science B.V. All rights reserved. | en |
dc.description.sponsorship | Universidad de Alcalá | es_ES |
dc.description.sponsorship | Comunidad de Madrid | es_ES |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | Elsevier | en |
dc.rights | (c) Elsevier Science, 1999 | |
dc.subject | Whey | en |
dc.subject | Soybean | en |
dc.subject | Food analysis | en |
dc.subject | Proteins | en |
dc.subject | Lactalbumin | en |
dc.subject | Lactoglobulins | en |
dc.title | Analysis of bovine whey proteins in soybean dairy-like products by capillary electrophoresis | en |
dc.type | info:eu-repo/semantics/article | en |
dc.subject.eciencia | Ciencia | es_ES |
dc.subject.eciencia | Química analítica e industrial | es_ES |
dc.subject.eciencia | Science | en |
dc.subject.eciencia | Chemistry, analytic and technical | en |
dc.contributor.affiliation | Universidad de Alcalá. Departamento de Química Analítica e Ingeniería Química | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/S0021-9673(99)00838-9 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dc.identifier.doi | 10.1016/S0021-9673(99)00838-9 | |
dc.relation.projectID | info:eu-repo/grantAgreement/UAH//E002%2F98/ES// | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/CAM//COR 0035%2F94/ES// | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/CAM//06G%2F047%2F96/ES// | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |