Characterization and differentiation of diverse transgenic and nontransgenic soybean varieties from CE protein profiles. Research article
Authors
García Ruiz, CarmenIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/1361DOI: 10.1002/elps.200600799
ISSN: 0173-0835
Publisher
John Wiley & Sons
Date
2007Bibliographic citation
Electrophoresis, 2007, v. 28, p. 2314-2323
Keywords
Pigmented soybean
Protein profiles
Transgenic soybean
Project
S-0505/AGR/0312 (Comunidad de Madrid)
AGL2005-05320-C02-01/ ALI (Ministerio de Ciencia y Tecnología)
RYC2003-001 (Ministerio de Ciencia y Tecnología)
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Publisher's version
http://dx.doi.org/10.1002/elps.200600799Rights
© WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, 2007
Access rights
info:eu-repo/semantics/openAccess
Abstract
Nowadays, soybeans are commercialized in a wide variety of colors and tones. Moreover,
some pigmented seeds are being commercialized as soybeans while, on other occasions,
these seeds are labeled as mung beans, azuki beans or soybean frijoles generating confusion
on their identity. In this work, CE has been applied for the first time for the characterization
and differentiation of different pigmented beans commercialized as soybeans.
Other seeds commercialized as azuki, mung green soybeans or soybean frijoles were also
analyzed. Borate buffer (at pH 8.5) containing 20% v/v ACN was used as the separation
media and solution containing ACN/water (75:25 v/v) with 0.3% v/v acetic acid was used to
solubilize the proteins from the samples. A 50 cm bare fused-silica capillary was employed
for obtaining adequate separations in about 12 min. The CE protein pattern observed for
yellow soybeans was different from that corresponding to green and red soybeans. The
seeds commercialized as black soybean presented electropherograms identical or similar to
those yielded by the yellow seeds with the exception of the sample labeled as black soybeans
frijoles that presented a totally different pattern. In addition, CE protein profiles obtained
for azuki and mung green soybeans were very similar to those corresponding to red soybeans
and green soybeans, respectively. Finally, the CE method was also applied to differentiate
transgenic and nontransgenic soybean varieties. Discriminant analysis, using several
protein peak areas as variable, was used to successfully classify these samples.
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