Development of a capillary electrophoresis method for the determination of soybean proteins in soybean-rice gluten-free dietary products. Research Article
Autores
García Ruiz, Carmen; García González, María Ángeles; García López, María Concepción; Marina Alegre, María LuisaIdentificadores
Enlace permanente (URI): http://hdl.handle.net/10017/1358DOI: 10.1002/elps.200500355
ISSN: 0173-0835
Editor
John Wiley & Sons
Fecha de publicación
2006Patrocinadores
Ministerio de Ciencia y Tecnología
Ministerio de Educación y Ciencia
Cita bibliográfica
Electrophoresis, 2006, v. 27, n. 2, p. 452-460
Palabras clave
Bakery products
Capillary electrophoresis
Gluten-free dietary products
Soybean proteins
Rice
Proyectos
info:eu-repo/grantAgreement/MINCYT//BQU2002–01199/ES/Desarrollo de métodos analíticos rápidos de cromatografía líquida de alta eficacia de perfusión y electroforesis capilar para la separación de proteínas vegetales
info:eu-repo/grantAgreement/MEC//RYC-2003-001/ES/RYC-2003-001/
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/publishedVersion
Versión del editor
http://dx.doi.org/10.1002/elps.200500355Derechos
© WILEY-VCH, 2006
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
CE has been applied for the first time to the simultaneous separation of soybean and
rice proteins. Treated and untreated capillaries with different effective lengths as well
as separation media at different pHs were tested. For that purpose, samples and
standard solutions were prepared in 25:75 ACN–water media containing 0.3% v/v
acetic acid. The use of an untreated capillary of 50 cm effective length together with an
80 mM borate buffer (pH 8.5) modified with 20% v/v ACN and UV detection at 254 nm
were the conditions working the best. These conditions enabled the determination of
soybean proteins in gluten-free dietary commercial products elaborated with soybean
protein and/or soybean flour and rice flour using the standard additions calibration
method. The method was linear up to 26 mg/mL of soybean proteins, the precision
(expressed as RSD) was always better than 6%, and recoveries obtained for soybean
proteins when spiking commercial products were very close to 100%
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