Rapid enantiomeric separation of polychlorinated biphenyls by electrokinetic chromatography using mixtures of neutral and charged cyclodextrin derivatives
Authors
García Ruiz, CarmenIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/1354DOI: 10.1016/S0021-9673(00)01176-6
ISSN: 0021-9673
Publisher
Elsevier
Date
2001Bibliographic citation
Journal of Chromatography A, 910 (2001) 157-164
Keywords
Enantiomer separation
Buffer composition
Polychlorinated biphenyls
Cyclodextrins
Project
07M/0049/1998 (Comunidad de Madrid)
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Publisher's version
http://dx.doi.org/10.1016/S0021-9673(00)01176-6Rights
© Elsevier, 2001
Access rights
info:eu-repo/semantics/openAccess
Abstract
Electrokinetic chromatography with cyclodextrin derivatives (CD-EKC) was used to achieve the rapid enantiomeric
separation of chiral polychlorinated biphenyls (PCBs). Thirteen of the 19 chiral PCBs stable at room temperature were individually separated into their two enantiomers by using 2-morpholinoethanesulfonic acid (MES) buffer (pH 6.5) containing carboxymethylated g-cyclodextrin (CM-g-CD) as pseudostationary phase mixed with b-cyclodextrin (b-CD) or permethylated b-cyclodextrin (PM-b-CD). Urea was also added to increase the solubility of PCBs and cyclodextrins in the aqueous separation buffer. Several experimental parameters such as the nature, concentration, and pH of the buffer, nature and concentration of the cyclodextrin derivatives used, and the addition of different additives were studied in order to improve the enantiomeric separation. In addition, the effect of some instrumental parameters such as separation temperature and applied voltage was also investigated. PCBs were enantiomerically separated in less than 12 min by using a 50 mM MES buffer (pH 6.5) containing 20 mM CM-g-CD, 10 mM b-CD or 20 mM PM-b-CD, and 2 M urea at a temperature of
458C and an applied voltage of 20 kV.
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Files | Size | Format |
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PCBs-J Chromatogr A, 910, ... | 204.0Kb |
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