Comparison of charged cyclodextrin derivatives for the chiral separation of atropisomeric polychlorinated biphenyls by capillary electrophoresis
Identifiers
Permanent link (URI): http://hdl.handle.net/10017/1353DOI: 10.1002/elps.200305465
ISSN: 0173-0835
Publisher
John Wiley & Sons
Date
2003Bibliographic citation
Electrophoresis, 2003, v. 24, p. 2657-2664
Keywords
Chiral capillary electrophoresis
Cyclodextrin derivatives
Polychlorinated biphenyls
Project
info:eu-repo/grantAgreement/CAM//07 M%2F0049%2F1998/ES//
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Publisher's version
http://dx.doi.org/10.1002/elps.200305465Rights
(c) WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, 2003
Access rights
info:eu-repo/semantics/openAccess
Abstract
Charged cyclodextrin (CD) derivatives were used as chiral selectors in electrokinetic
chromatography (EKC) for the chiral separation of highlyhydrophobic neutral racemates
such as atropisomeric polychlorinated biphenyls (PCBs). -CD-phosphated, -CD sulfated,
succinylated--CD (Succ--CD) and succinylated- -CD (Succ- -CD) were used
as anionic CDs. As cationic CD, 6-monodeoxy-6-monoamino- -CD ( -CD-NH2) was
tested for the first time in order to separate PCBs. From the different CD derivatives
employed, the best separations were obtained with the cationic CD derivative. Thus,
the use of -CD-NH2 in phosphate buffer at pH 2.0 containing urea allowed the chiral
recognition of eleven PCBs (45, 84, 88, 91, 95, 131, 136, 144, 149, 176, and 197). In this
case, the addition of 2 M urea to the buffer solution was crucial to achieve the chiral
separation of PCBs. The addition of acetonitrile to 10 mM phosphate buffer (pH 2.0)
with 30 mM -CD-NH2 and 2 M urea improved considerably the chiral resolution obtained
for PCBs 91, 95, 136, 144, 149, and 197 although an increase in the analysis time was
also observed. All the results obtained were compared with those previously obtained
with the dual CD system carboxymethyl--CD/ -CD.
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