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dc.contributor.authorKuijt, J.
dc.contributor.authorGarcía Ruiz, Carmen 
dc.contributor.authorStroomberg, G.J.
dc.contributor.authorMarina Alegre, María Luisa 
dc.contributor.authorAriese, Freek
dc.contributor.authorBrinkman, U.A.Th.
dc.contributor.authorGooijera, C.
dc.date.accessioned2008-03-03T08:38:23Z
dc.date.available2008-03-03T08:38:23Z
dc.date.issued2001
dc.identifier.bibliographicCitationJournal of Chromatography A, 2001, v. 907, p. 291-299en
dc.identifier.issn0021-9673
dc.identifier.urihttp://hdl.handle.net/10017/1352
dc.description.abstractThe separation of five phenolic polycyclic aromatic hydrocarbon metabolites (hydroxy-PAHs) has been performed by cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) using a 30 mM borate buffer (pH 9.0) containing 60 mM sodium dodecyl sulfate and varying concentrations of γ-cyclodextrin (γ-CD). A concentration of 12.5 mM γ-CD was found to provide a baseline separation of the five hydroxy-PAHs. We applied conventional fluorescence and laser-induced fluorescence (LIF) detection, using a new, small-size, quadrupled Nd–YAG laser emitting at 266 nm. The best limits of detection, in the low ng/ml range, were achieved using LIF detection. For all analytes, linearity was observed up to ca. 100 ng/ml. As an application, conjugated pyrene metabolites in hepatopancreas samples from the terrestrial isopods Oniscus asellus and Porcellio scaber were separated and detected. Finally, flatfish bile samples from individuals exposed to polluted sediment or crude oil, which were part of an interlaboratory study, were analyzed by CD-MEKC with conventional fluorescence and LIF detection to determine the 1-hydroxypyrene concentrations.en
dc.description.sponsorshipThe authors wish to thank the Dutch Foundation for the Advancement of Science (NOW) for financial support and equipment (grant No. 344-006). Also, the technical assistance of Mr. J. Buijs is much appreciated.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherElsevieren
dc.rights(c) Elsevier, 2001en
dc.subjectLaser-induced fluorescence detectionen
dc.subjectDetection, electrophoresisen
dc.subjectPolynuclear aromatic hydrocarbonsen
dc.titleLaser-induced fluorescence detection at 266 nm in capillary electrophoresis Polycyclic aromatic hydrocarbon metabolites in biotaen
dc.typeinfo:eu-repo/semantics/articleen
dc.subject.ecienciaCienciaes_ES
dc.subject.ecienciaQuímica analítica e industriales_ES
dc.subject.ecienciaScienceen
dc.subject.ecienciaChemistry, analytic and technicalen
dc.contributor.affiliationUniversidad de Alcalá. Departamento de Química Analítica e Ingeniería Química
dc.relation.publisherversionhttp://dx.doi.org/10.1016/S0021-9673(00)01040-2
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.identifier.doi10.1016/S0021-9673(00)01040-2
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen


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