Laser-induced fluorescence detection at 266 nm in capillary electrophoresis Polycyclic aromatic hydrocarbon metabolites in biota

Authors
Kuijt, J.; García Ruiz, CarmenUniversity of Alcalá Author; Stroomberg, G.J.; Marina Alegre, María LuisaUniversity of Alcalá Author; Ariese, Freek; [et al.]
Identifiers
Permanent link (URI): http://hdl.handle.net/10017/1352
DOI: 10.1016/S0021-9673(00)01040-2
ISSN: 0021-9673
Publisher
Elsevier
Date
2001
Affiliation
Universidad de Alcalá. Departamento de Química Analítica e Ingeniería Química
Funders
The authors wish to thank the Dutch Foundation for the Advancement of Science (NOW) for financial support and equipment (grant No. 344-006). Also, the technical assistance of Mr. J. Buijs is much appreciated.
Bibliographic citation
Journal of Chromatography A, 2001, v. 907, p. 291-299
Keywords
Laser-induced fluorescence detection
Detection, electrophoresis
Polynuclear aromatic hydrocarbons
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Publisher's version
http://dx.doi.org/10.1016/S0021-9673(00)01040-2
Rights
(c) Elsevier, 2001
Access rights
info:eu-repo/semantics/openAccess
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Abstract
The separation of five phenolic polycyclic aromatic hydrocarbon metabolites (hydroxy-PAHs) has been performed by cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) using a 30 mM borate buffer (pH 9.0) containing 60 mM sodium dodecyl sulfate and varying concentrations of γ-cyclodextrin (γ-CD). A concentration of 12.5 mM γ-CD was found to provide a baseline separation of the five hydroxy-PAHs. We applied conventional fluorescence and laser-induced fluorescence (LIF) detection, using a new, small-size, quadrupled Nd–YAG laser emitting at 266 nm. The best limits of detection, in the low ng/ml range, were achieved using LIF detection. For all analytes, linearity was observed up to ca. 100 ng/ml. As an application, conjugated pyrene metabolites in hepatopancreas samples from the terrestrial isopods Oniscus asellus and Porcellio scaber were separated and detected. Finally, flatfish bile samples from individuals exposed to polluted sediment or crude oil, which were part of an interlaboratory study, were analyzed by CD-MEKC with conventional fluorescence and LIF detection to determine the 1-hydroxypyrene concentrations.
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