Retention modeling and resolution optimization for a group of N-phenylpyrazole derivatives in micellar electrokinetic chromatography using empirical and physicochemical models
IdentifiersPermanent link (URI): http://hdl.handle.net/10017/1349
John Wiley & Sons
The authors thank the Comisión Interministerial de Ciencia y Tecnología (Spain) for project PB98-0709 and Dr. O. Jiménez thanks the Universidad de Alcalá (Madrid,Spain) for project E029/98.
Electrophoresis, 2003, v. 24, p. 325-335
Micellar electrokinetic chromatography
PB98-0709 (Comisión Interministerial de Ciencia y Tecnología)
E029/98 (Universidad de Alcalá)
(c) Wiley, 2003
The optimization of the separation resolution for a group of N-phenylpyrazole derivatives in micellar electrokinetic chromatography (MEKC) as a function of the separation buffer composition (surfactant and organic modifier concentration) has been performed. In order to achieve our purpose, the first step has been the prediction of the migration times of the electroosmotic flow (t0) and micelles (tm), and the retention factors of solutes (k), as a function of surfactant (sodium dodecyl sulfate) and alcohol (n-propanol or n-butanol) concentrations, by means of empirical equations. Also, some physicochemical models have been applied to relate the retention factors to the surfactant and the organic modifier concentrations in order to optimize the separation resolution and to increase our knowledge of the separation process. Finally, a comparison of the resolution optimization through the use of the physicochemical and empirical models selected has been made in order to obtain the optimum separation buffer composition for the separation of a group of 17 N-phenylpyrazole derivatives as test solutes.
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