Monitoring the stereoselectivity of biodegradation of chiral polychlorinated biphenyls using electrokinetic chromatography
Authors
García Ruiz, CarmenIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/1328DOI: 10.1002/1615-9314(20020101)25:1/2<17::AID-JSSC17>3.0.CO;2-F
ISSN: 1615-9306
Publisher
John Wiley & Sons
Date
2002Funders
The authors thank the Comunidad Autónoma de Madrid (Spain) for projects 07 M/0640/1997 and 07 M/0049/1998.
Bibliographic citation
Journal of Separation Science, 2002, v. 25, p. 17-22
Keywords
Chiral polychlorinated biphenyls
Stereoselectivity
Biodegradation
Electrokinetic chromatograph
Project
07 M_0640_1997 (Comunidad de Madrid)
07 M_0049_1998 (Comunidad de Madrid)
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Publisher's version
http://dx.doi.org/10.1002/1615-9314(20020101)25:1/2<17::AID-JSSC17>3.0.CO;2-FRights
(c) Wiley, 2002
Access rights
info:eu-repo/semantics/openAccess
Abstract
A study of the biodegradation of several chiral polychlorinated biphenyls (PCBs,
IUPAC numbers 45, 88, 91, 95, 136, 144, 149, and 176) by a naturally occurring soil
bacterium as well as the stereoselectivity of such process has been performed using
Electrokinetic Chromatography with cyclodextrins (CD-EKC). In order to achieve the
analysis of chiral PCBs, a CD-EKC system based on 50 mM 2-morpholinoethanesulfonic
acid (MES) buffer (pH 6.5) containing 2 M urea, 20 mM carboxymethylated ccyclodextrin
(CM-c-CD), and 10 mM b-cyclodextrin (b-CD) was used. First, the percentage
degradation of PCBs by the microorganism used in this work was measured
for different incubation times (22, 46, 71, 93, 165, 213, and 262 hours). The results
showed a high degree of biodegradation (from 61% to 94%) for the eight PCBs studied
after 262 hours of incubation with the microorganism, degradation being favoured
for the least chlorinated congeners. Secondly, measurement of the atropisomeric
ratio of the PCB atropisomers during the biodegradation process revealed no significant
variation of the atropisomeric ratios with the degradation time, indicating a nonstereoselective
degradation of PCBs by the microorganism
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Biodegradation PCBs-J Sep Sci, ... | 87.75Kb |
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