Unidad docente Especialidades médicasESPECMEDhttp://hdl.handle.net/10017/2462024-03-29T12:43:00Z2024-03-29T12:43:00ZEfficacy of antimicrobial agents delivered to hernia meshes using an adaptable thermo-responsive hyaluronic acid-based coatingPérez Köhler, BárbaraLinardi, FlavioPascual González, María GemmaBellón Caneiro, Juan ManuelEglin, DavidGuillaume, Olivierhttp://hdl.handle.net/10017/597862024-01-24T01:16:38Z2020-01-01T00:00:00ZEfficacy of antimicrobial agents delivered to hernia meshes using an adaptable thermo-responsive hyaluronic acid-based coating
Pérez Köhler, Bárbara; Linardi, Flavio; Pascual González, María Gemma; Bellón Caneiro, Juan Manuel; Eglin, David; Guillaume, Olivier
Purpose Mesh-related infection is a critical outcome for patients with hernia defect stabilized with synthetic or biological meshes. Even though bioactive meshes loaded with antibiotics or antiseptics are slowly emerging in the market, the available solutions still lack versatility. Here, we proposed a polymer solution, i.e., hyaluronic acid-poly(N-isopropylacrylamide) (HApN), which forms a hydrogel to be used as coating for meshes only when it reaches body temperature. Methods We assessed how the gelation of HApN was influenced by the incorporation of different antibiotic and antiseptic formulations, and how this gel can be used to coat several mesh types. The impact of the coating on the elastic behavior of a macroporous mesh was tested under cyclic elongation condition. Finally, we selected two different coating formulations, one based on antibiotics (gentamicin + rifampicin) and one based on antiseptic (chlorhexidine) and tested in vitro their antimicrobial efficacies. Results HApN can be used as carrier for different antimicrobial agents, without having a strong influence on its gelation behavior. Porous or dense meshes can be coated with this polymer, even though the stability was not optimal on macroporous meshes such as Optilene when pores are too large. HApN loaded with drugs inhibited in vitro the growth of several Gram-positive and Gram-negative bacteria. Conclusion Compared to the available technologies developed to endow meshes with antibacterial activity, the proposed HApN offers further versatility with potential to prevent mesh-related infection in hernioplasty.
19 p.
2020-01-01T00:00:00ZBehaviour at the peritoneal interface of next generation prosthetic materials for hernia repairBenito Martínez, SelmaPascual González, María GemmaRodríguez Mancheño, MartaPérez-Köhler, BárbaraGarcía-Moreno Nisa, FranciscaBellón Caneiro, Juan Manuelhttp://hdl.handle.net/10017/596082024-03-11T16:16:41Z2022-01-01T00:00:00ZBehaviour at the peritoneal interface of next generation prosthetic materials for hernia repair
Benito Martínez, Selma; Pascual González, María Gemma; Rodríguez Mancheño, Marta; Pérez-Köhler, Bárbara; García-Moreno Nisa, Francisca; Bellón Caneiro, Juan Manuel
Background When using a prosthetic material in hernia repair, the behaviour of the mesh at the peritoneal interface is especially important for implant success. Biomaterials developed for their intraperitoneal placement are known as composites and are made up of two different-structure materials, one is responsible for good integration within host tissue and the other is responsible to make contact with the viscera. This study examines the behaviour at the peritoneal level of two composites, the fully degradable Phasix-ST® and the partially degradable Symbotex®. A polypropylene mesh (Optilene®) served as control. Methods Sequential laparoscopy from 3 to 90 days, in a preclinical model in the New Zealand white rabbit, allowed monitoring adhesion formation. Morphological studies were performed to analyse the neoperitoneum formed in the repair process. Total macrophages were identified by immunohistochemical labelling. To identify the different macrophage phenotypes, complementary DNAs were amplified by qRT-PCR using specific primers for M1 (TNF-?/CXCL9) and M2 (MRC1/IL-10) macrophages. Results The percentage of firm and integrated adhesions remained very high in the control group over time. Both composites showed a significant decrease in adhesions at all study times and in qualitative terms were mainly loose. Significant differences were also observed from 7 days onwards between the two composites, increasing the values in Phasix over time. Neoperitoneum thickness for Phasix was significantly greater than those of the other meshes, showing mature and organized neoformed connective tissue. Immunohistochemically, a significantly higher percentage of macrophages was observed in Symbotex. mRNA expression levels for the M2 repair-type macrophages were highest for Phasix but significant differences only emerged for IL-10. Conclusions Fewer adhesions formed to the Symbotex than Phasix implants. Ninety days after implant, total macrophage counts were significantly higher for Symbotex, yet Phasix showed the greater expression of M2 markers related to the tissue repair process.
22 p.
2022-01-01T00:00:00ZAntibacterial polypropylene mesh fixation with a cyanoacrylate adhesive improves its response to infectionBenito Martínez, SelmaPérez Köhler, BárbaraRodríguez Mancheño, MartaGarcía-Moreno Nisa, FranciscaGómez Gil, VerónicaPascual González, María GemmaBellón Caneiro, Juan Manuelhttp://hdl.handle.net/10017/596032024-03-11T16:13:59Z2021-01-01T00:00:00ZAntibacterial polypropylene mesh fixation with a cyanoacrylate adhesive improves its response to infection
Benito Martínez, Selma; Pérez Köhler, Bárbara; Rodríguez Mancheño, Marta; García-Moreno Nisa, Francisca; Gómez Gil, Verónica; Pascual González, María Gemma; Bellón Caneiro, Juan Manuel
Background: Antibacterial meshes for hernia repair seek to avoid infection in the patient. As thesebiomaterials are especially prone to bacteria settling at their sutured borders, this study examines whether the use of a cyanoacrylate tissue adhesive could improve mesh behavior at the fixation zones. Methods: First, antibacterial polypropylene meshes were prepared by soaking in 0.05% chlorhexidine, and the response of n-hexyl cyanoacrylate to contamination with Staphylococcus aureus ATCC25923 was assessed in vitro. Then, in a preclinical model, partial defects (5 x 3 cm) were created in the abdominal wall of 18 New Zealand White rabbits and repaired with mesh to establish the following 3 study groups: (1) mesh without chlorhexidine fixed with cyanoacrylate, (2) antibacterial mesh fixed with sutures, and (3) antibacterial mesh fixed with cyanoacrylate (n = 6 each). The implants were inoculated with 106 CFU/ mL of S aureus. At 14 days after surgery, bacterial adhesion to the implant and its integration within host tissue were determined through microbiological, histological and immunohistochemical procedures. Results: As observed in vitro, the cyanoacrylate gave rise to a 1.5-cm bacteria-free margin around the prosthetic mesh. In vivo, the tissue adhesive prevented bacterial adhesion to the fixation zones, reducing infection of chlorhexidine-free meshes and optimizing the efficacy of the antibacterial meshes compared with those fixed with sutures. Conclusion: These findings indicated that cyanoacrylate fixation does not affect mesh integration into the host tissue. Likewise, the antibacterial behavior and tissue response of a chlorhexidine-treated polypropylene mesh is improved when cyanoacrylate is used for its fixation.
22 p.
2021-01-01T00:00:00ZWound Healing Modulation through the Local Application of Powder Collagen-Derived Treatments in an Excisional Cutaneous Murine ModelBenito Martínez, SelmaPérez Köhler, BárbaraRodríguez Mancheño, MartaIzco, Jesús MaríaRecalde, José IgnacioPascual González, María Gemmahttp://hdl.handle.net/10017/596012024-03-08T16:51:19Z2022-01-01T00:00:00ZWound Healing Modulation through the Local Application of Powder Collagen-Derived Treatments in an Excisional Cutaneous Murine Model
Benito Martínez, Selma; Pérez Köhler, Bárbara; Rodríguez Mancheño, Marta; Izco, Jesús María; Recalde, José Ignacio; Pascual González, María Gemma
Wound healing includes dynamic processes grouped into three overlapping phases: inflammatory, proliferative, and maturation/remodeling. Collagen is a critical component of a healing wound and, due to its properties, is of great interest in regenerative medicine. This preclinical study was designed to compare the effects of a new collagen-based hydrolysate powder on wound repair to a commercial non-hydrolysate product, in a murine model of cutaneous healing. Circular excisional defects were created on the dorsal skin of Wistar rats (n = 36). Three study groups were established according to the treatment administered. Animals were euthanized after 7 and 18 days. Morphometric and morphological studies were performed to evaluate the healing process. The new collagen treatment led to the smallest open wound area throughout most of the study. After seven days, wound morphometry, contraction, and epithelialization were similar in all groups. Treated animals showed reduced granulation tissue formation and fewer inflammatory cells, and induction of vasculature with respect to untreated animals. After 18 days, animals treated with the new collagen treatment showed accelerated wound closure, significantly increased epithelialization, and more organized repair tissue. Our findings suggest that the new collagen treatment, compared to the untreated control group, produces significantly faster wound closure and, at the same time, promotes a slight progression of the reparative process compared with the rest of the groups.
15 p.
2022-01-01T00:00:00Z