%0 Journal Article %A Marina Alegre, María Luisa %A Plaza del Moral, Merichel %A Vásquez Villanueva, Romy Ángela %A García López, María Concepción %T Recovery and determination of cholesterol-lowering compounds from Olea europaea seeds employing pressurized liquid extraction and gas chromatography-mass spectrometry %D 2020 %@ 0026-265X %U http://hdl.handle.net/10017/44501 %X Our previous work demonstrated the presence of compounds with hypocholesterolemic capacity in olive seeds. These compounds were extracted using CO2-expanded ethyl acetate and identified as tyrosol, hydroxytyrosol, and beta-sitosterol using GC&#-MS. This work describes the extraction of these compounds from different olive seeds using pressurized ethyl acetate. Their solubility in ethyl acetate at temperatures ranging from 40&-200 °C was theoretically predicted by Hansen solubility parameters. The content of these compounds was estimated by GC&#-MS, as well as, the reduction of the micellar cholesterol solubility (RMCS) capacity of extracts enabling to establish the optimum extraction temperature at 100 °C. A GC&-MS method was developed and validated in terms of its analytical characteristics for a sensitive determination and quantification of tyrosol, hydroxytyrosol, and &;946#sitosterol in different olive seeds. Within varieties, Manzanilla seeds presented the highest concentration of tyrosol, hydroxytyrosol, and the lowest concentration of beta-sitosterol, as well as the highest RMCS capacity %K Cholesterol-lowering capacity %K Hansen solubility parameters %K Olive seed %K Phenolic compounds %K Phytosterols %K Pressurized liquid extraction %K Química %K Chemistry %~ Biblioteca Universidad de Alcala