%0 Journal Article %A Xu, Dongsheng %A Shao, Huikai %A Luo, Rongying %A Wang, Qiqin %A Sánchez López, Elena %A Fanali, Salvatore %A Marina Alegre, María Luisa %A Jiang, Z. %T A facile and efficient single-step approach for the fabrication of vancomycin functionalized polymer-based monolith as chiral stationary phase for nano-liquid chromatography %D 2018 %@ 0021-9673 %U http://hdl.handle.net/10017/41506 %X A facile single-step preparation strategy for fabricating vancomycin functionalized organic polymer based monolith within 100 mu m fused-silica capillary was developed. The synthetic chiral functional monomer, i.e 2-isocyanatoethyl methacrylate (ICNEML) derivative of vancomycin, was co-polymerized with the cross-linker ethylene dimethacrylate (EDMA) in the presence of methanol and dimethyl sulfoxide as the selected porogens. The co-polymerization conditions were systematically optimized in order to obtain satisfactory column performance. Adequate permeability, stability and column morphology were observed for the optimized poly(ICNEML-vancomycin-co-EDMA) monolith. A series of chiral drugs were evaluated on the monolith in either several other beta-blockers. The proposed single-step approach not only resulted in a vancomycin functionalized organer polar organic-phase or reversed-phase modes. After the optimization of separation conditions, baseline or partial enantioseparation were obtained for series of drugs including thalidomide, colchicine, carteolol, salbutamol, clenbuterol andic polymer-based monolith with acceptable performance, but also significantly simplified the preparation procedure by reducing time and labor. %K Vancomycin %K Enantioseparation %K Organic polymeric monolith %K Nano-LC %K Química %K Chemistry %~ Biblioteca Universidad de Alcala