%0 Journal Article 
%A Martínez Girón, Ana Belén
%A Domínguez Vega, Elena
%A García Ruiz, Carmen
%A Crego Navazo, Antonio Luis
%A Marina Alegre, María Luisa
%T Enantiomeric separation of ornithine in complex mixtures of amino acids by EKC with off-line derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate
%D 2008
%@ 1570-0232
%U http://hdl.handle.net/10017/3831
%X A new analytical methodology was developed by EKC enabling the fast enantiomeric separation of
Ornithine in complex mixtures of amino acids. A previous derivatization step with 6-aminoquinolyl-Nhydroxysuccinimidyl
carbamate (AQC) was achieved to enable the sensitive UV detection of amino acids
as well as to make possible their interaction with the CDs employed as chiral selectors. A dual CD system
containing an anionic and a neutral CD in phosphate buffer at acid pH showed a high resolving power
allowing the enantiomeric separation of 18 protein amino acids and Orn. The method was applied to the
analysis of fermented foods to investigate the extent of the presence of Orn enantiomers.
%K Ornithine
%K Chiral separation
%K Cyclodextrins
%K AQC derivatization
%K Amino acids
%K Fermented foods
%K Electrokinetic chromatography
%K Ciencia
%K Química analítica e industrial
%K Science
%K Chemistry, analytic and technical
%~ Biblioteca Universidad de Alcala