%0 Journal Article 
%A Marina Alegre, María Luisa
%A García López, María Concepción
%A Vásquez Villanueva, Romy
%T Identification by hydrophilic interaction and reversed-phase liquid chromatography-tandem mass spectrometry of peptides with antioxidant capacity in food residues
%D 2016
%@ 0021-9673
%U http://hdl.handle.net/10017/24608
%X HILIC- and RP-HPLC-ESI-Q-TOF identification of bioactive peptides with antioxidant capacity in peach by-products was carried out. Peach seeds contain more than 40% of proteins (as dried and defatted basis) and could constitute a cheap source of bioactive peptides. Extracted proteins were digested using four different commercial enzymes. Five assays based on different antioxidant mechanisms were employed for a reliable evaluation of the antioxidant capacity of the extracts. Thermolysin enzyme originated the extract with the most favorable antioxidant capacity. Probably due to a synergic effect among antioxidant peptides, it was not possible to find a peptide fraction with a higher antioxidant capacity than the whole extract. Eighteen peptides were identified in the whole hydrolysate when combining HILIC- and RP-HPLC-ESI-Q-TOF. A high percentage of hydrophobic amino acids were observed within their sequences which is a characteristic feature of the antioxidant nature of peptides.
%K Hydrophilic interaction
%K Reversed-phase
%K Liquid chromatography–tandem mass spectrometry
%K Peptides
%K Antioxidant
%K Peach seed
%K CIENCIA
%K Química
%K SCIENCE
%K Chemistry
%~ Biblioteca Universidad de Alcala