%0 Journal Article 
%A Sánchez Hernández, Laura
%A Castro Puyana, María
%A Marina Alegre, María Luisa
%A Crego Navazo, Antonio Luis
%T Determination of betaines in vegetable oils by capillary electrophoresis tandem mass spectrometry. Application to the detection of olive oil adulteration with seed oils.
%D 2011
%@ 0173-0835
%U http://hdl.handle.net/10017/21130
%X A capillary electrophoresis-tandem mass spectrometry methodology enabling the
simultaneous determination of betaines (glycine betaine, trigonelline, proline betaine and
total content of carnitines) in vegetable oils was developed. Betaines were derivatized
with butanol previous to their baseline separation in 10 min using a 0.1 M formic acid
buffer at pH 2.0. Ion trap conditions were optimized in order to maximize selectivity and
sensitivity. Analytical characteristics of the proposed method were established by
evaluating its selectivity, linearity, precision (RSDs ranged from 4.8% to 10.7% for
corrected peak areas), accuracy by means of recovery studies (from 80% to 99%) and
LODs and LOQs at 0.1 ppb level. The method was applied to the determination of the
selected betaines in seed oils and extra virgin olive oils. MS2 experiments provided the
fingerprint fragmentation for all betaines studied in seed oils. In extra virgin olive oils,
carnitines were not detected being possible to propose them as a feasible novel marker for
the detection of adulterations of olive oils. Application of the developed method to the
analysis of different mixtures of extra virgin olive oil with seed oil (between 2-10 %)
enabled the detection and quantitation of the total content of carnitines. The results
obtained show the high potential of the developed method for the authentication and
quality control of olive oils.
%K Betaines
%K Capillary electrophoresis
%K Corn oil
%K Olive oil
%K Soybean oil
%K Sunflower oil
%K Tandem mass spectrometry
%K Ciencia
%K Química
%K Science
%K Chemistry
%~ Biblioteca Universidad de Alcala